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After 18 hours, cells were labelled with 10 nM JFX646 Halo ligand (kind gift from the Lavis lab, Janelia) in optiMEM (Gibco) for 3 minutes, followed by 3 washes in mKRB buffer (140 mM NaCl, 2.8 mM KCl, 2 mM MgCl 2 , 1 mM CaCl 2 , 10 mM HEPES, 10 mM glucose, pH 7.4) at 37 C

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The intersection of regulatory change and economic pressure creates an unprecedented opportunity to help individuals and families build health savings while managing Bronze plan costs, Dr